PI/RNase Staining Buffer
產(chǎn)品名稱: PI/RNase Staining Buffer
產(chǎn)品型號(hào): BD-550825
產(chǎn)品展商: BD Pharmingen
產(chǎn)品文檔: 無(wú)相關(guān)文檔
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PI/RNase Staining Buffer
的詳細(xì)介紹
產(chǎn)品名稱:PI/RNase Staining Buffer
PI/RNase Staining Buffer 產(chǎn)品介紹:
Material Number: 550825
Size: 100 ml
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
PI/RNase Staining Buffer Description:
Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by
digestion with ribonucleases. The content of DNA as determined by flow cytometry, can reveal useful information about the cell cycle and the
proteins involved in the regulation of the cell cycle. Cells in G2 and M phases of the cell cycle have double the DNA content of those in G0
and G1 phases. Cells in S phase have a DNA content lying between these extremes. PI is detected in the orange range of the spectrum using a
562-588 nm band pass filter. This reagent may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for
examining the expression of proteins during the cell cycle.
PI/RNase Staining Buffer Preparation and Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Do not dilute or boil.
PI/RNase Staining Buffer Suggested Companion Products:
Catalog Number Name Size Clone
554656 Stain Buffer (FBS) 500 ml (none)
554655 Fixation Buffer 100 ml (none)
558052 Perm Buffer II 125 ml (none)
558050 Perm Buffer III 125 ml (none)